[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Antigenic maps of influenza A/H3N2 virus produced with human antisera obtained after primary infection [ ]
Judith M. Fonville 1,2,3,*, Pieter L. A. Fraaij 3,4, Gerrie de Mutsert 3, Samuel H. Wilks 1,2, Ruud van Beek 3, Ron A. M. Fouchier 3 and Guus F. Rimmelzwaan 3
Author Affiliations: 1Centre for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom 2WHO Collaborating Center for Modeling, Evolution, and Control of Emerging Infectious Diseases, Cambridge, United Kingdom 3Department of Viroscience, Erasmus MC, 3015 CE Rotterdam, The Netherlands 4Department of Pediatrics, Erasmus MC-Sophia, 3015 CN Rotterdam, The Netherlands
*Corresponding author contact information: Dr. Judith M Fonville; Center for Pathogen Evolution, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, United Kingdom. Fax: +44 1223336676; telephone +44 1223330933+44 1223330933; email: firstname.lastname@example.org
Antigenic characterization of influenza viruses is typically based on hemagglutination inhibition (HI) assay data of viral isolates tested against strain-specific post-infection ferret antisera. Here, similar virus characterizations were performed using first-infection human rather than ferret serology data.
We screened sera collected between 1995 and 2011 from children between 9 and 24 months of age for influenza virus antibodies, performed HI tests for the positive sera against 24 influenza viruses isolated between 1989 and 2011, and measured HI titers of 24 A/H3N2 ferret sera against the same panel of viruses.
Of the 17 positive human sera, 6 were high-responders, showing HI patterns that would be expected from primary infection antisera, while 11 sera had lower, more dispersed patterns of reactivity that are not easily explained. The antigenic map based on the high-responder human HI data was similar to the results using ferret data.
Although the overall structure of the ferret and human antigenic maps is similar, local differences in virus positions indicate that the human and ferret immune system might see antigenic properties of viruses differently. Further studies are needed to establish to what degree of detail ferret data provide equivalent patterns to human serological reactivity.
© The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.
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